Search results for "BDNA test"

showing 3 items of 3 documents

HCV viraemia is more important than genotype as a predictor of response to interferon in sicily (Southern Italy)

1996

Abstract Background/Aims: To investigate host- and virus-related factors predictive of early and sustained alanine aminotransferase normalization after interferon therapy for HCV-related chronic liver disease, in an area where genotype 1 is highly prevalent. Methods: We studied 100 patients with HCV-RNA positive chronic liver disease (73 chronic hepatitis and 27 cirrhosis) undergoing alpha-interferon treatment. Thirty-four patients had an early response but relapsed, 15 patients remained into sustained response for at least 12 months after therapy, and 51 patients did not respond. Serum HCV-RNA levels were assessed by bDNA (Chiron), and genotype by LiPA (Innogenetics) and by sequencing of t…

AdultLiver CirrhosisMaleCirrhosisGenotypeHepatitis C virusMolecular Sequence DataAlpha interferonChronic liver diseasemedicine.disease_causeAntiviral AgentsGenotypePrevalenceBDNA testmedicineHumansViremiaSicilyInterferon alfaBase SequenceHepatologybusiness.industryInterferon-alphaAlanine TransaminaseHepatitis CMiddle Agedmedicine.diseaseHepatitis CTreatment OutcomeLiverChronic DiseaseImmunologyNucleic Acid ConformationFemalebusinessmedicine.drugJournal of Hepatology
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All-trans retinoic acid for treatment of chronic hepatitis C

2008

Background/Aims: In vitro studies in the subgenomic hepatitis C virus (HCV) replicon system have identified all-trans retinoic acid (ATRA) as a potential therapeutic against hepatitis C. Thus, the antiviral potential of this drug should be assessed in vivo. Methods: Twenty highly treatment experienced serotype 1 patients with non-response to conventional or pegylated interferon-α (Peg-/IFN-α) and ribavirin were randomly assigned to 12 weeks of monotherapy with ATRA (group A) or a combination of ATRA and PegIFN-α2a (group B). HCV RNA was assessed by bDNA assay and if negative by highly sensitive polymerase chain reaction. Results: During treatment, five of 10 patients in group A had a drop o…

HepatitisHepatologybiologyorganic chemicalsRibavirinHepacivirusHepatitis C virusvirus diseasesHepatitis CPharmacologymedicine.diseasebiology.organism_classificationmedicine.disease_causeVirologybiological factorschemistry.chemical_compoundchemistryPegylated interferonmedicineBDNA testneoplasmsViral loadmedicine.drugLiver International
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Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues

2020

The current methods to study the distribution and dynamics of viral RNA molecules inside infected cells are not ideal, as electron microscopy and immunohistochemistry can only detect mature virions, and quantitative real-time PCR does not reveal localized distribution of RNAs. We demonstrated here the branched DNA in situ hybridization (bDNA ISH) technology to study both the amount and location of the emerging &minus

0301 basic medicineMicrobiology (medical)replication030106 microbiologyCellIn situ hybridizationBiologybranched DNAmedicine.disease_causeinfektiotMicrobiologyArticleantiviral drugs03 medical and health scienceschemistry.chemical_compoundpositive RNAVirologymedicineBDNA testlcsh:QH301-705.5replikaatioenterovirusvirus diseasesRNATranslation (biology)negative RNAVirologyenterovirukset030104 developmental biologymedicine.anatomical_structurelcsh:Biology (General)chemistryVirion assemblyRNAEnterovirusin situ hybridization3111 BiomedicineDNAMicroorganisms
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